An improved method for preparing thick sections for immuno/histochemistry and confocal microscopy and its use to identify rare events.

@article{Monaghan2001AnIM,
  title={An improved method for preparing thick sections for immuno/histochemistry and confocal microscopy and its use to identify rare events.},
  author={Paul F. Monaghan and Patricia R. Watson and Harold G. Cook and Lucy C. Scott and Timothy S. Wallis and David Professor Robertson},
  journal={Journal of microscopy},
  year={2001},
  volume={203 Pt 2},
  pages={
          223-6
        }
}
Detection of rare events within solid tissues by immunocytochemistry is aided by imaging thick sections. Sections of 40--100 microm thickness of paraformaldehyde-fixed solid tissue can be prepared by use of a vibrating microtome and when immunolabelled these sections can be imaged in a confocal microscope. This approach provides excellent preservation of the structure of the sample and imposes minimal antigenic damage. In studies of the invasion of the bovine intestinal epithelium by Salmonella… CONTINUE READING
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