An improved method for preparing Agrobacterium cells that simplifies the Arabidopsis transformation protocol

@article{Logemann2006AnIM,
  title={An improved method for preparing Agrobacterium cells that simplifies the Arabidopsis transformation protocol},
  author={Elke Logemann and Rainer P. Birkenbihl and Bekir {\"U}lker and Imre E Somssich},
  journal={Plant Methods},
  year={2006},
  volume={2},
  pages={16 - 16}
}
The Agrobacterium vacuum (Bechtold et al 1993) and floral-dip (Clough and Bent 1998) are very efficient methods for generating transgenic Arabidopsis plants. These methods allow plant transformation without the need for tissue culture. Large volumes of bacterial cultures grown in liquid media are necessary for both of these transformation methods. This limits the number of transformations that can be done at a given time due to the need for expensive large shakers and limited space on them… CONTINUE READING

From This Paper

Topics from this paper.
40 Citations
5 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 40 extracted citations

References

Publications referenced by this paper.
Showing 1-5 of 5 references

In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants

  • N Bechtold, J Ellis, G Pelletier
  • C R Acad Sci Paris, Life Sciences
  • 1993
1 Excerpt

Simplified Arabidopsis Transformation Protocol

  • SJ Clough, AF Bent

Similar Papers

Loading similar papers…