An improved method for preparation, stabilization, and storage of house fly (Diptera: Muscidae) microsomes.

Abstract

An improved method for preparation and storage of insect microsomes from house fly, Musca domestica L., abdomens was developed. Microsomes were prepared in phosphate buffer fortified with glycerol, dithiothreitol, ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, and 1-phenyl-2-thiourea. No cytochrome P-420 was observed when abdomens were isolated by our method. No measurable loss of cytochrome P-450, cytochrome b5 or NADPH-cytochrome c (P-450) reductase levels, or methoxyresorufin O-demethylation, 7-ethoxycoumarin O-deethylation, or aryl hydrocarbon hydroxylation activities occurred when a diluted suspension (protein concentration of 2 mg/ml) of microsomes was stored at -80 degrees C for at least 2 mo.

Cite this paper

@article{Lee1989AnIM, title={An improved method for preparation, stabilization, and storage of house fly (Diptera: Muscidae) microsomes.}, author={Scott S Lee and Jeffrey G Scott}, journal={Journal of economic entomology}, year={1989}, volume={82 6}, pages={1559-63} }