An improved method for generating and identifying human induced pluripotent stem cells.

@article{Mali2010AnIM,
  title={An improved method for generating and identifying human induced pluripotent stem cells.},
  author={Prashant Mali and Zhaohui Ye and Bin-Kuan Chou and Jonathan Yen and Linzhao Cheng},
  journal={Methods in molecular biology},
  year={2010},
  volume={636},
  pages={191-205}
}
This chapter describes the methods we use to derive and characterize human induced pluripotent stem (iPS) cells. We describe in order, first our culture techniques for the starting fibroblast populations and methods for retrovirus preparation and concentration. Subsequently, a detailed iPS derivation protocol suitable for human fibroblast populations is discussed using standard retroviral vectors expressing the classic four or three reprogramming genes. Finally, we elaborate a robust technique… CONTINUE READING

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An Improved Method for Generating and Identifying Human Induced Pluripotent Stem GQ

  • IH Park, R Zhao, +6 authors Daley
  • Nature
  • 2008

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