An improved long-term culture of rat hepatocytes to detect liver tumour-promoting agents: results with phenobarbital.

@article{Mesnil1993AnIL,
  title={An improved long-term culture of rat hepatocytes to detect liver tumour-promoting agents: results with phenobarbital.},
  author={Marc Mesnil and Colette Piccoli and Hiroshi Yamasaki},
  journal={European journal of pharmacology},
  year={1993},
  volume={248 1},
  pages={59-66}
}
Among various cocultures of hepatocytes with other cell types, we found that mouse embryonal cells (BALB/c 3T3) are more effective in maintaining rat hepatocytes in vitro. Because most human cancers are epithelial in origin, we thought that such a hepatocyte culture system could be used for the detection of tumour-promoting agents, most of which are inhibitors of gap-junctional intercellular communication. We, therefore, have examined the effect of the strong rat liver tumour promoter… CONTINUE READING