An improved high-throughput Nile red fluorescence assay for estimating intracellular lipids in a variety of yeast species.

@article{Sitepu2012AnIH,
  title={An improved high-throughput Nile red fluorescence assay for estimating intracellular lipids in a variety of yeast species.},
  author={Irnayuli R. Sitepu and Laura Ignatia and Annaliese K Franz and Diana M Wong and Sarah Asih Faulina and Ming Tsui and Atit Kanti and Kyria Boundy-Mills},
  journal={Journal of microbiological methods},
  year={2012},
  volume={91 2},
  pages={321-8}
}
A rapid and inexpensive method for estimating lipid content of yeasts is needed for screening large numbers of yeasts samples. Nile red is a fluorescent lipophilic dye used for detection and quantification of intracellular lipid droplets in various biological system including algae, yeasts and filamentous fungi. However, a published assay for yeast is affected by variable diffusion across the cell membrane, and variation in the time required to reach maximal fluorescence emission. In this study… CONTINUE READING