An improved extraction procedure for an HPLC method for cyclosporin A in whole blood and plasma.

Abstract

Cyclosporin A (CyA) was assayed by high performance liquid chromatography (HPLC) following extraction of the whole blood or plasma with diethyl ether and chromatography on a Sep-Pak Silica cartridge. Using carefully chosen volumes and ratios of ethyl acetate and hexane in the eluting sequences on the Sep-Pak, the major interferent present in normal blood and serum was eliminated.

Cite this paper

@article{Maguire1987AnIE, title={An improved extraction procedure for an HPLC method for cyclosporin A in whole blood and plasma.}, author={Susan P Maguire and F Kyne and Diarmuid UaConaill}, journal={Annals of clinical biochemistry}, year={1987}, volume={24 ( Pt 2)}, pages={161-6} }