An improved cryofixation method: cryoquenching of small tissue blocks during microwave irradiation.

@article{Hanyu1992AnIC,
  title={An improved cryofixation method: cryoquenching of small tissue blocks during microwave irradiation.},
  author={Yuji Hanyu and Masumi Ichikawa and Gen Matsumoto},
  journal={Journal of microscopy},
  year={1992},
  volume={165 Pt 2},
  pages={255-71}
}
The metal contact method of rapid freezing is greatly improved by irradiating the specimen with microwaves at 2.45 GHz for a short period of time (50 ms), while pushing the specimen onto the surface of the copper block cooled by liquid N2. The microwave irradiation, together with two technical improvements (a light-mass plunger and a recently developed beta-gel shock absorber) for preventing bounce, produces a good freezing zone for squid retina, with high reproducibility for each experimental… CONTINUE READING
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