An improved colony hybridization method with significantly increased sensitivity for detection of single genes.

@article{Maas1983AnIC,
  title={An improved colony hybridization method with significantly increased sensitivity for detection of single genes.},
  author={Renata Maas},
  journal={Plasmid},
  year={1983},
  volume={10 3},
  pages={296-8}
}
By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sensitivity, probably by increasing the total denatured plasmid DNA fixed to the paper.