An improved chromogenic substrate endotoxin assay for clinical use.

@article{Harris1983AnIC,
  title={An improved chromogenic substrate endotoxin assay for clinical use.},
  author={Richard I. Harris and Philip Crapse Stone and James Stuart},
  journal={Journal of clinical pathology},
  year={1983},
  volume={36 10},
  pages={1145-9}
}
An improved quantitative assay for endotoxin in plasma was developed after evaluating three different chromogenic substrates and seven methods for removal of plasma inhibitors. Optimal storage conditions for plasma samples prior to assay were also determined. Using chromogenic substrate S2423 with plasma diluted 1/10 in water and heated to 75 degrees C for 5 min to remove inhibitors, a within-batch coefficient of variation of 4% was obtained at levels of endotoxin likely to be encountered… CONTINUE READING