An improved automated determination of serum total cholesterol with a single color reagent.

@article{Block1966AnIA,
  title={An improved automated determination of serum total cholesterol with a single color reagent.},
  author={Walter D. Block and K J Jarrett and John B. Levine},
  journal={Clinical chemistry},
  year={1966},
  volume={12 10},
  pages={681-9}
}
An anhydrous color reagent containing ferric chloride is pumped through Tygon tubing to a glass coil in a 950 heating bath. Manually prepared serum extracts (1:20 isopropanol dilution) are presented to the stream of preheated color reagent, and the two are passed through three mixing coils, connected in series. The absorbance of the resultant color is determined at 550 m in a 15 mm. tubular flow-cell. The improved N-automated procedure gave values of greater precision (5.6%) for total… CONTINUE READING