An improved and highly standardised transformation procedure allows efficient production of single and multiple targeted gene-knockouts in a moss, Physcomitrella patens

@article{Hohe2003AnIA,
  title={An improved and highly standardised transformation procedure allows efficient production of single and multiple targeted gene-knockouts in a moss, Physcomitrella patens},
  author={A. Hohe and T. Egener and J. M. Lucht and H. Holtorf and C. Reinhard and G. Schween and R. Reski},
  journal={Current Genetics},
  year={2003},
  volume={44},
  pages={339-347}
}
Abstract The moss Physcomitrella patens is the only land plant known to date with highly efficient homologous recombination in its nuclear DNA, making it a unique model for plant functional genomics approaches. For high-throughput production of knockout plants, a robust transformation system based on polyethylene glycol-mediated transfection of protoplasts was developed and optimised. Both the DNA conformation and pre-culture of plants used for protoplast isolation significantly affected… Expand
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