An improved, efficient method for analyzing human sperm chromosomes using zona-free hamster ova.

@article{Kamiguchi1986AnIE,
  title={An improved, efficient method for analyzing human sperm chromosomes using zona-free hamster ova.},
  author={Yujiroh Kamiguchi and Kazuya Mikamo},
  journal={American journal of human genetics},
  year={1986},
  volume={38 5},
  pages={724-40}
}
We have developed an improved method for analyzing human sperm chromosome, using zona-free hamster ova. Our main improvements of methodology are as follows: (1) Fertilization rate of hamster oocytes by human spermatozoa was markedly raised by successive treatments of the spermatozoa with 5-15 microM ionophore A23187 solutions and a capacitation medium (BWW medium) containing 3.5% HSA. The HSA most effective in inducing capacitation was selected from several kinds of HSA products commercially… CONTINUE READING