An imprinted transcript, antisense to Nesp, adds complexity to the cluster of imprinted genes at the mouse Gnas locus.

@article{Wroe2000AnIT,
  title={An imprinted transcript, antisense to Nesp, adds complexity to the cluster of imprinted genes at the mouse Gnas locus.},
  author={Sarah Wroe and Gavin Kelsey and Judith A. Skinner and D Bodle and Simon T. Ball and Colin V. Beechey and Josephine Peters and Christine M. Williamson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 7},
  pages={
          3342-6
        }
}
  • S. Wroe, G. Kelsey, +5 authors C. Williamson
  • Published 2000
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
The Gnas locus in distal mouse chromosome (Chr) 2 is emerging as a complex genomic region. It contains three imprinted genes in the order Nesp-Gnasxl-Gnas. Gnas encodes a G protein alpha-subunit, and Nesp and Gnasxl encode proteins of unknown function expressed in neuroendocrine tissue. Together, these genes form a single transcription unit because transcripts of Nesp and Gnasxl are alternatively spliced onto exon 2 of Gnas. Nesp and Gnasxl are expressed from opposite parental alleles, with… Expand
Alternative non-coding splice variants of Nespas, an imprinted gene antisense to Nesp in the Gnas imprinting cluster
TLDR
The identification of a set of small spliced imprinted transcripts in the human and now in the mouse suggests that these antisense transcripts are functionally important. Expand
A comprehensive transcript map of the mouse Gnas imprinted complex.
TLDR
Two clones extended Nespas in the 3' direction, providing evidence of antisense transcription spanning a 30-kb genomic region from a single allele, and a pattern of alternate splicing was revealed by the Gnas transcript map by the FANTOM2 clones. Expand
Epigenetic Properties and Identification of an Imprint Mark in the Nesp-Gnasxl Domain of the Mouse Gnas Imprinted Locus
TLDR
The Gnas locus in the mouse is imprinted with a complex arrangement of alternative transcripts defined by promoters with different patterns of monoallelic expression, and models to account for the regulation of imprinting at the locus are proposed. Expand
Identification of an imprinting control region affecting the expression of all transcripts in the Gnas cluster
TLDR
It is established that a paternally derived targeted deletion of the germline differentially methylated region (DMR) associated with the antisense Nespas transcript unexpectedly affects both the expression of all transcripts in the Gnas cluster and methylation of two DMRs. Expand
Identification of a Methylation Imprint Mark within the Mouse Gnas Locus
TLDR
Differential methylation in this region is established during gametogenesis, being present in oocytes and absent in spermatozoa, and is maintained in preimplantation E3.5d blastocysts, which may be important for the tissue-specific imprinting of GSα. Expand
Control of imprinting at the Gnas cluster.
TLDR
Targeted deletion analysis is performed to test candidate regulatory regions within the Gnas complex and, unlike other imprinted domains, two major control regions have been identified. Expand
Control of Imprinting at the Gnas Cluster
TLDR
Targeted deletion analysis is performed to test candidate regulatory regions within the Gnas complex and, unlike other imprinted domains, two major control regions have been identified that controls the imprinted expression of a single transcript and is subsidiary to and must interact with, a principal control region that affects the expression of all transcripts. Expand
A cis-acting control region is required exclusively for the tissue-specific imprinting of Gnas
TLDR
It is established that the differentially methylated region at exon 1A contains an imprinting control element that specifically regulates Gnas and comprises a characterized ICR for a gene that is only weakly imprinted in a minority of tissues. Expand
Imprinting the Gnas locus
TLDR
This review considers how imprinting of Gnas was discovered, the phenotypic consequences of mutations in each of the gene products, both in the mouse and human, and some conjectures to explain why this elaborate imprinted locus has evolved in this manner in mammals. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 26 REFERENCES
A cluster of oppositely imprinted transcripts at the Gnas locus in the distal imprinting region of mouse chromosome 2.
  • J. Peters, S. Wroe, +5 authors G. Kelsey
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1999
TLDR
Gnasxl, Nesp, and Gnas are all part of the same transcription unit; transcripts for Gnasxl and Nesp are alternatively spliced onto exon 2 of Gnas, demonstrating an imprinting mechanism in which two oppositely imprinted genes share the same downstream exons. Expand
Bidirectional imprinting of a single gene: GNAS1 encodes maternally, paternally, and biallelically derived proteins.
TLDR
This work investigates the allelic origin of other mRNAs derived from GNAS1, a gene showing simultaneous imprinting in both the paternal and maternal directions, and finds this gene to be remarkable in the complexity of its allele-specific regulation. Expand
A novel imprinted gene, encoding a RING zinc-finger protein, and overlapping antisense transcript in the Prader-Willi syndrome critical region.
TLDR
ZNF127 and ZNF127AS are novel imprinted genes that may be associated with some of the clinical features of the polygenic Prader-Willi syndrome. Expand
The human GNAS1 gene is imprinted and encodes distinct paternally and biallelically expressed G proteins.
TLDR
It is shown that, although Gsalpha expression (directed by the promoter upstream of exon 1) is biallelic, GNAS1 is indeed imprinted in a promoter-specific fashion, and may contribute to the anomalous inheritance of PHP Ia. Expand
LIT1, an imprinted antisense RNA in the human KvLQT1 locus identified by screening for differentially expressed transcripts using monochromosomal hybrids.
TLDR
An extensive screen for differentially expressed transcripts in the 11p15 region was performed using monochromosomal hybrids with a paternal or maternal human chromosome 11 to identify an imprinted antisense transcript identified within the KvLQT1 locus, which is associated with multiple balanced chromosomal rearrangements in BWS and an additional breakpoint in embryonal rhabdoid tumors. Expand
Multiple imprinted sense and antisense transcripts, differential methylation and tandem repeats in a putative imprinting control region upstream of mouse Igf2.
TLDR
The existence of multiple, overlapping imprinted (maternally repressed) sense and antisense transcripts that are associated with a tandem repeat in the mouse Igf2 upstream region supports the proposal that tandem repeats act to target methylation to imprinted genetic loci. Expand
Identification of imprinted loci by methylation-sensitive representational difference analysis: application to mouse distal chromosome 2.
TLDR
Representational difference analysis (RDA) is used to clone HpaII fragments with methylation differences on the maternal and paternal copies of distal chromosome (Chr) 2 in the mouse, leading to the recovery of multiple differentially methylated Hpa II fragments at two major sites of imprinted methylation. Expand
Imprinting of a RING zinc-finger encoding gene in the mouse chromosome region homologous to the Prader-Willi syndrome genetic region.
TLDR
Zfp127 is a novel imprinted gene that may play a role in the imprinted phenotype of mouse models of PWS, and it is hypothesize that the gametic imprint may be set, at least in part, by the transcriptional activity of Zfp127 in pre- and post-meiotic male germ cells. Expand
Imprinted expression of the Igf2r gene depends on an intronic CpG island
TLDR
A primary role for region 2 is defined and a negligible role for chromosomal location is defined in Igf2r imprinting; they show that methylation imprints can maintain allelic expression and that expression competition could play a general role in imprinting. Expand
Glomerular-specific imprinting of the mouse gsalpha gene: how does this relate to hormone resistance in albright hereditary osteodystrophy?
TLDR
Results strongly suggest that Gnas is maternally imprinted and suggest that the mouse gene may be imprinted in a manner opposite that predicted in human. Expand
...
1
2
3
...