An immunoprotective monoclonal antibody directed against Leptospira interrogans serovar copenhageni.

@article{Schoone1989AnIM,
  title={An immunoprotective monoclonal antibody directed against Leptospira interrogans serovar copenhageni.},
  author={Gerard J. Schoone and C. O. R. Everard and H. Korver and D. G. Carrington and V A Inniss and Jean Baulu and Wiepko J. Terpstra},
  journal={Journal of general microbiology},
  year={1989},
  volume={135 1},
  pages={
          73-8
        }
}
A monoclonal antibody (mAb) was prepared by hybridoma technology in BALB/c mice immunized to Leptospira interrogans serovar copenhageni. This mAb agglutinated serovars copenhageni and icterohaemorrhagiae to high titres and protected hamsters, dogs and monkeys against challenge with a virulent strain of serovar copenhageni. The mAb gave protection to hamsters at dilutions up to 1 in 1000; at a 1 in 10 dilution the protective effect lasted for at least two weeks. Biochemical analysis by SDS-PAGE… 
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References

SHOWING 1-10 OF 27 REFERENCES
A monoclonal antibody reacting with a determinant on leptospiral lipopolysaccharide protects guinea pigs against leptospirosis.
TLDR
Daily administration of purified MUM/F1-1/copenhageni IgA before and after challenge with 2 X 10(8) virulent homologous leptospires passively protected newborn guinea pigs against lethal leptposirosis.
Serological and protective-antibody responses of rabbits to leptospiral antigens.
TLDR
The rabbit antibody response to leptospiral F4 antigen extracted from serovar pomona depended on the method of immunisation, which stimulated agglutinins in both the IgM and IgG classes.
The classification of Sejroe group serovars of Leptospira interrogans with monoclonal antibodies.
Comparative studies on two antigens (F4 and TM) extracted from leptospires
TLDR
F4 andTM antigens extracted from Leptospira interrogans serovars pomona, icterohaemorrhagiae, and copenhageni were compared by immunodiffusion, by hemagglutination inhibition, and by selective absorption of antisera to suggest that F4 and TM are different antigen.
Prevention of renal carriage of leptospirosis in dogs by vaccination
Dogs vaccinated with a Leptospira interrogans vaccine containing serogroups Canicola and Icterohaemorrhagiae and prepared from cultures grown in a protein-free medium resisted challenge with…
Comparative classification of Leptospira serovars of the Pomona group by monoclonal antibodies and restriction-endonuclease analysis.
  • W. Terpstra, H. Korver, +4 authors A. Kolk
  • Biology
    Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology
  • 1987
Immunogenicity of Whole Cell and Outer Envelope Leptospiral Vaccines in Hamsters
TLDR
The OE from virulent strains of these four serotypes plus hardjo were combined to form a pentavalent OE vaccine which proved to be satisfactory in immunogenic potency and duration of immunity.
A new leptospiral vaccine for use in man. II. Clinical and serologic evaluation of a field trial with volunteers.
TLDR
The new vaccine, consisting of killed Leptospira, the only one grown in a chemically defined medium, was innocuous, and led to seroconversion in 57% of all volunteers who received two doses of the vaccine.
Isolation of an antigenic oligosaccharide fraction from Leptospira interrogans serovar canicola with a monoclonal antibody.
An oligosaccharide fraction containing the antigenic determinant of lipopolysaccharide antigen (TM antigen) from Leptospira interrogans serovar canicola, recognized by a monoclonal antibody (CT3)…
Monoclonal antibodies that protect in vivo against Plasmodium chabaudi recognize a 250,000-dalton parasite polypeptide
TLDR
The results suggest that this 250,000-dalton malaria parasite antigen may be important in the induction and expression of antibody-mediated immunity to malaria.
...
1
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3
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