An immunochromatographic strip sensor for sildenafil and its analogues

@inproceedings{Xu2019AnIS,
  title={An immunochromatographic strip sensor for sildenafil and its analogues},
  author={Chuanlai Xu and Zhongxing Wang and Xiaoling Wu and Liu Liqiang and Liguang Xu},
  year={2019}
}
In this study, a new hapten of sildenafil (Sild) was successfully synthesized and a sensitive monoclonal antibody (mAb) against Sild was produced based on this new hapten. The subclass of the mAb was IgG2a, and the half-maximal inhibitory concentration (IC50) of the mAb was 0.53 ng mL−1. Next, an immunochromatographic assay (ICA) was established for detecting Sild and its analogues in functional foods, where the visual detection limit (vLOD) and cut-off values were 0.5 and 20 μg kg−1… CONTINUE READING

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