Cells dissociated from embryonic rat septum were transfected with SV40 large T antigen gene by the calcium phosphate precipitation method. One of the cloned lines (EG6) had a flat morphology and expressed nestin gene, a marker of neural stem cells. Upon stimulation with basic fibroblast growth factor (bFGF), morphological changes of EG6 cells were induced, e.g. shrinkage of cell body and extension of neuritic processes. Furthermore, bFGF induced expression of the high affinity NGF receptor (trkA) gene. EG6 cell line thus will be useful for studies of roles of bFGF in the developing CNS as well as for studies of the regulation of trkA gene expression.