An extranuclear expression system for analysis of cytoplasmic promoters of yeast linear killer plasmids.

@article{Schrnder1995AnEE,
  title={An extranuclear expression system for analysis of cytoplasmic promoters of yeast linear killer plasmids.},
  author={J{\"u}rgen Schr{\"u}nder and Friedhelm Meinhardt},
  journal={Plasmid},
  year={1995},
  volume={33 2},
  pages={
          139-51
        }
}
Based on the cytoplasmically localized killer plasmids pGKL1 and pGKL2 of Kluyveromyces lactis two new linear hybrid plasmids were constructed which consist of pGKL1, into which in addition to the previously developed cytoplasmically expressible LEU2* selectable marker a glucose dehydrogenase-encoding bacterial gene (gdh A) has been integrated. One of the hybrid plasmids carries the bacterial gene preceded by an arbitrarily placed cytoplasmic promoter (upstream conserved sequence) in front of… CONTINUE READING
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