An extra-plastidial alpha-glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not necessary for transient starch degradation.

@article{Glaring2007AnEA,
  title={An extra-plastidial alpha-glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not necessary for transient starch degradation.},
  author={Mikkel A Glaring and Agnieszka Zygadlo and David Thorneycroft and Alexander Schulz and Steven M Smith and Andreas Blennow and Lone Baunsgaard},
  journal={Journal of experimental botany},
  year={2007},
  volume={58 14},
  pages={3949-60}
}
Starch phosphorylation catalysed by the alpha-glucan, water dikinases (GWD) has profound effects on starch degradation in plants. The Arabidopsis thaliana genome encodes three isoforms of GWD, two of which are localized in the chloroplast and are involved in the degradation of transient starch. The third isoform, termed AtGWD2 (At4g24450), was heterologously expressed and purified and shown to have a substrate preference similar to potato GWD. Analyses of AtGWD2 null mutants did not reveal any… CONTINUE READING