An extended RNA/RNA duplex structure within the coding region of mRNA does not block translational elongation.

@article{Lingelbach1988AnER,
  title={An extended RNA/RNA duplex structure within the coding region of mRNA does not block translational elongation.},
  author={Klaus R. Lingelbach and Bernhard Dobberstein},
  journal={Nucleic acids research},
  year={1988},
  volume={16 8},
  pages={3405-14}
}
RNA/RNA duplex formation involving the 5'untranslated region of a mRNA can efficiently block translation. Here we investigated the effect on translation of an RNA/RNA duplex between part of the coding region and sequences of the 3'untranslated region of lysozyme mRNA. A cDNA was constructed which contained 2 identical sequences of 150 nucleotides, one of which was an inverted repeat of the other. Cell-free transcription of this cDNA with T7 RNA polymerase resulted in a mRNA with an extended RNA… CONTINUE READING