An expression vector for the phytopathogenic fungus, Ustilago maydis.

@article{Kinal1991AnEV,
  title={An expression vector for the phytopathogenic fungus, Ustilago maydis.},
  author={H H Kinal and Jihan Tao and J. A. Bruenn},
  journal={Gene},
  year={1991},
  volume={98 1},
  pages={129-34}
}
We have constructed an expression vector for the phytopathogenic fungus Ustilago maydis. This vector, pUXV, expresses genes located downstream from a U. maydis glyceraldehyde-3-phosphate dehydrogenase promoter. Plasmid pUXV also contains a selective marker gene conferring resistance to the antibiotic hygromycin B and a U. maydis autonomously replicating sequence, UARS, allowing high transformation efficiency. Expression of a cDNA from the toxin-encoding region of the U. maydis virus P6 in pUXV… CONTINUE READING