An exploration of ambigrammatic sequences in narnaviruses

@article{Derisi2019AnEO,
  title={An exploration of ambigrammatic sequences in narnaviruses},
  author={Joseph L. Derisi and Greg Huber and Amy L Kistler and Hanna Retallack and Michael Wilkinson and David Yllanes},
  journal={Scientific Reports},
  year={2019},
  volume={9}
}
Narnaviruses have been described as positive-sense RNA viruses with a remarkably simple genome of ~3 kb, encoding only a highly conserved RNA-dependent RNA polymerase (RdRp). Many narnaviruses, however, are ‘ambigrammatic’ and harbour an additional uninterrupted open reading frame (ORF) covering almost the entire length of the reverse complement strand. No function has been described for this ORF, yet the absence of stops is conserved across diverse narnaviruses, and in every case the codons in… 
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Analysis of Robin polymorphisms suggests that its reverse open reading frame also does not code for a functional protein, suggesting that its role in ambigrammatic narnavirus genomes is unclear.
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