An experimental comparison of the maximum likelihood estimation and nonlinear least-squares fluorescence lifetime analysis of single molecules.

@article{Maus2001AnEC,
  title={An experimental comparison of the maximum likelihood estimation and nonlinear least-squares fluorescence lifetime analysis of single molecules.},
  author={Michael Maus and Mircea Cotlet and Johan Hofkens and Thomas Gensch and Frans C. De Schryver and Joerg Schaffer and Claus A M Seidel},
  journal={Analytical chemistry},
  year={2001},
  volume={73 9},
  pages={2078-86}
}
Two procedures based on the weighted least-squares (LS) and the maximum likelihood estimation (MLE) method to confidently analyze single-molecule (SM) fluorescence decays with a total number (N) of 2,500-60,000 counts have been elucidated and experimentally compared by analyzing measured bulk and SM decays. The key observation of this comparison is that the LS systematically underestimates the fluorescence lifetimes by approximately 5%, for the range of 1,000-20,000 events, whereas the MLE… CONTINUE READING
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