An experiment with glycerol-frozen red blood cells stored at -80 degrees C for up to 37 years.

@article{Valeri2000AnEW,
  title={An experiment with glycerol-frozen red blood cells stored at -80 degrees C for up to 37 years.},
  author={C. Robert Valeri and Gina M Ragno and Linda E Pivacek and George P Cassidy and Rithy Srey and M Hansson-Wicher and M E Leavy},
  journal={Vox sanguinis},
  year={2000},
  volume={79 3},
  pages={168-74}
}
BACKGROUND AND OBJECTIVES Red cells frozen using 40% W/V glycerol are currently FDA approved for frozen storage at -80 degrees C for up to 10 years. MATERIALS AND METHODS Red cells frozen with 40% W/V glycerol and stored at -80 degrees C for up to 37 years were thawed, deglycerolized, and stored at 4 degrees C for 24 h. RESULTS Red cells frozen for up to 37 years had mean freeze-thaw-wash recovery values of 75%, less than 1% hemolysis, and normal ATP, 2,3-DPG and P50 levels, and 60% of… CONTINUE READING

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