An exo- -N-acetylglucosaminidase from Bacillus subtilis B; characterization.

@article{Berkeley1973AnE,
  title={An exo- -N-acetylglucosaminidase from Bacillus subtilis B; characterization.},
  author={R. C. Berkeley and Stephen James Brewer and Jos{\'e} Manuel Burgos Ort{\'i}z and James B. Gillespie},
  journal={Biochimica et biophysica acta},
  year={1973},
  volume={309 1},
  pages={
          157-68
        }
}
The highly purified exo-β-N-acetylglucosaminidase (β-2-acetamido-2-deoxy-d-glucoside acetamidodeoxyglucohydrolase (EC 3.2.1.30) of Bacillus subtilis B has a pH Optimum of 5.9 and is most stable at 8.5. Its isoelectric point appears to be pH 3.8 and it has a mol. wt of about 75 000. The results of studies on its ability to attack a wide range of synthetic and natural substrates are given and kinetic data for the hydrolysis of a number of these reported. The Km and V values for p-nitrophenyl-2… CONTINUE READING