An examination of methods for extraction of soil phospholipids

  title={An examination of methods for extraction of soil phospholipids},
  author={C. Kowalenko and R. McKercher},
  journal={Soil Biology \& Biochemistry},
Abstract Three methods for measurement of soil phospholipid material were compared for their relative efficiencies. When applied to a few Saskatchewan soils, the Hance and Anderson (1963) method, and a modification employing hexane-acctone (1:1, v/v) as the extractant and a more vigorous but shorter shaking period, indicated phospholipid contents of 3–5 ppm P. A third method (Bligh and Dyer, 1959) gave lower values (about 2 ppm). Reproducibility of results was highest with the modified Hance… Expand