An evaluation of the activation of endogenous pancreatic enzymes during human islet isolations.

@article{Rose2003AnEO,
  title={An evaluation of the activation of endogenous pancreatic enzymes during human islet isolations.},
  author={Natisha L Rose and Monica Palcic and A M James Shapiro and Jonathan Rt Lakey},
  journal={Transplantation proceedings},
  year={2003},
  volume={35 7},
  pages={2455-7}
}
Despite advances in human islet isolations, there remain inconsistencies in human islet yield and viability after collagenase digestion. It has been suggested that trypsin may contribute to the proteolysis of collagenase and the destruction of islet cells, or possibly exert indirect effects on the pancreas by activating other endogenous serine proenzymes. This study evaluated the effects of serine proteases on collagenase activity and profiled the kinetics of serine protease activity throughout… CONTINUE READING