An evaluation of target specificity and sensitivity of three qPCR assays for detecting environmental DNA from Northern Pike (Esox lucius)

@article{Olsen2015AnEO,
  title={An evaluation of target specificity and sensitivity of three qPCR assays for detecting environmental DNA from Northern Pike (Esox lucius)},
  author={Jeffrey B. Olsen and Cara J. Lewis and Robert L. Massengill and Kristine J. Dunker and John K. Wenburg},
  journal={Conservation Genetics Resources},
  year={2015},
  volume={7},
  pages={615-617}
}
We developed and evaluated three qPCR assays for detecting environmental DNA from northern pike (Esox lucius). The assays target the cytochrome oxidase 1 (EluCOI), control region (EluCR), and cytochrome b (EluCytB) genes of the mitochondrial DNA. Target specificity, assessed using the fluorescence signal (at 45 cycles) to noise (at 1 cycle) ratio (S/N… CONTINUE READING