An evaluation of Feulgen-acriflavine-SO2 and Hoechst 33258 for DNA cytofluorometry in tumour pathology

@article{Bjelkenkrantz1983AnEO,
  title={An evaluation of Feulgen-acriflavine-SO2 and Hoechst 33258 for DNA cytofluorometry in tumour pathology},
  author={Kaj Bjelkenkrantz},
  journal={Histochemistry},
  year={1983},
  volume={79},
  pages={177-191}
}
The performance of Feulgen-acriflavine-SO2 and Hoechst 33258 for cytofluorometric ploidy determination is evaluated and compared. The fast initial fading of acriflavine-SO2 stained nuclei, with substantial reduction in fluorescence intensity within about 10 ms, is shown to be related to changes in DNA-ratios between cell types when measurements are performed using different excitation light intensities. Although low intensity excitation-, without such fading-, was used, the acriflavine-SO2… CONTINUE READING
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