An estimate of the crosstalk matrix in four-dye fluorescence-based DNA sequencing.

@article{Li1999AnEO,
  title={An estimate of the crosstalk matrix in four-dye fluorescence-based DNA sequencing.},
  author={Liang Li and Terence P. Speed},
  journal={Electrophoresis},
  year={1999},
  volume={20 7},
  pages={1433-42}
}
Color separation is an essential step of the data processing in the four-dye fluorescence detection strategy used in automated DNA sequencing. In this paper, we propose a model to describe the crosstalk phenomenon, and show how the assumptions of the model are supported by experimental data. The crosstalk matrix is estimated via a reparameterization based on a mapping between the distribution of fluorescence intensities and that of dye concentrations. An iterative algorithm is designed to… CONTINUE READING

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