An essential role for the baseplate protein Gp45 in phage adsorption to Staphylococcus aureus

@inproceedings{Li2016AnER,
  title={An essential role for the baseplate protein Gp45 in phage adsorption to Staphylococcus aureus},
  author={Xuehua Li and Cengiz Koç and Petra K{\"u}hner and York-Dieter Stierhof and Bernhard Krismer and Mark C. Enright and Jos{\'e} R Penad{\'e}s and Christiane Wolz and Thilo Stehle and Christian Cambillau and Andreas Peschel and Guoqing Xia},
  booktitle={Scientific reports},
  year={2016}
}
Despite the importance of phages in driving horizontal gene transfer (HGT) among pathogenic bacteria, the underlying molecular mechanisms mediating phage adsorption to S. aureus are still unclear. Phage ϕ11 is a siphovirus with a high transducing efficiency. Here, we show that the tail protein Gp45 localized within the ϕ11 baseplate. Phage ϕ11 was efficiently neutralized by anti-Gp45 serum, and its adsorption to host cells was inhibited by recombinant Gp45 in a dose-dependent manner. Flow… CONTINUE READING