An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes.

@article{Semple2006AnER,
  title={An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes.},
  author={Jeffrey W. Semple and Lance F Da-Silva and Eric J. Jervis and Jennifer Ah-Kee and Hyder Al-Attar and Lutz Kummer and John J. Heikkila and Philippe Pasero and Bernard P. Duncker},
  journal={The EMBO journal},
  year={2006},
  volume={25 21},
  pages={5150-8}
}
The heterohexameric origin recognition complex (ORC) acts as a scaffold for the G(1) phase assembly of pre-replicative complexes (pre-RC). Only the Orc1-5 subunits appear to be required for origin binding in budding yeast, yet Orc6 is an essential protein for cell proliferation. Imaging of Orc6-YFP in live cells revealed a punctate pattern consistent with the organization of replication origins into subnuclear foci. Orc6 was not detected at the site of division between mother and daughter cells… CONTINUE READING