An erythromycin process improvement using the diethyl methylmalonate-responsive (Dmr) phenotype of the Saccharopolyspora erythraea mutB strain

@article{Weber2011AnEP,
  title={An erythromycin process improvement using the diethyl methylmalonate-responsive (Dmr) phenotype of the Saccharopolyspora erythraea mutB strain},
  author={J. Mark Weber and William H. Cernota and Melissa C. Gonzalez and Benjamin I. Leach and Andrew R. Reeves and Roy K. Wesley},
  journal={Applied Microbiology and Biotechnology},
  year={2011},
  volume={93},
  pages={1575-1583}
}
The Saccharopolyspora erythraea mutB knockout strain, FL2281, having a block in the methylmalonyl-CoA mutase reaction, was found to carry a diethyl methylmalonate-responsive (Dmr) phenotype in an oil-based fermentation medium. The Dmr phenotype confers the ability to increase erythromycin A (erythromycin) production from 250–300% when the oil-based medium is supplemented with 15 mM levels of this solvent. Lower concentrations of the solvent stimulated proportionately less erythromycin… 
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