We have previously shown that two K+ must bind to cause the conformational change in Mg(2+)-dependent and Na(+)- and K(+)-stimulated ATPase reported by fluorescein 5'-isothiocyanate modification and that the ratio of the macroscopic K+ dissociation constants is 4 [Smirnova, I. N., & Faller, L. D. (1993) J. Biol. Chem. 268, 16120-16123]. Since 4 is the ratio… (More)
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