An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo.

@article{Jenke2002AnER,
  title={An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo.},
  author={Bok Hee C Jenke and Christian P Fetzer and Isa M. Stehle and Franziska J{\"o}nsson and Frank O Fackelmayer and Harald S. Conradt and J{\"u}rgen Bode and Hans Joachim Lipps},
  journal={EMBO reports},
  year={2002},
  volume={3 4},
  pages={349-54}
}
pEPI-1, a vector in which a chromosomal scaffold/matrix-attached region (S/MAR) is linked to the simian virus 40 origin of replication, is propagated episomally in CHO cells in the absence of the virally encoded large T-antigen and is stably maintained in the absence of selection pressure. It has been suggested that mitotic stability is provided by a specific interaction of this vector with components of the nuclear matrix. We studied the interactions of pEPI-1 by crosslinking with cis… CONTINUE READING

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The Hitchhiking Principle: optimizing episomal vectors for use in gene therapy and biotechnology

  • J. Bode, C. P. Fetzer, +6 authors H. J. Lipps
  • Int. J. Gene Ther. Mol. Biol.,
  • 2001
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