An enzyme-linked immunosorbent assay to detect antibodies against glycoprotein gE of bovine herpesvirus 1 allows differentiation between infected and vaccinated cattle.

@article{Oirschot1997AnEI,
  title={An enzyme-linked immunosorbent assay to detect antibodies against glycoprotein gE of bovine herpesvirus 1 allows differentiation between infected and vaccinated cattle.},
  author={J T van Oirschot and Marianne Kaashoek and Mieke A Maris-Veldhuis and Klaas Weerdmeester and Frans A. M. Rijsewijk},
  journal={Journal of virological methods},
  year={1997},
  volume={67 1},
  pages={23-34}
}
A blocking enzyme-linked immunosorbent assay (ELISA) was developed for detecting antibodies against glycoprotein gE (gE) of bovine herpesvirus 1 (BHV1). The assay is based on the use of two monoclonal antibodies directed against different antigenic domains on gE. Sera from uninfected cattle and cattle that had been repeatedly vaccinated with gE-negative marker vaccines scored negative, whereas sera from cattle naturally or experimentally infected with BHV1 field strains scored positive in the… CONTINUE READING

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