An enzyme-linked immunosorbent assay for hippuric acid: its potential application for biological monitoring of toluene exposure.

@article{Inagaki1994AnEI,
  title={An enzyme-linked immunosorbent assay for hippuric acid: its potential application for biological monitoring of toluene exposure.},
  author={Hirofumi Inagaki and Masayasu Minami},
  journal={International archives of occupational and environmental health},
  year={1994},
  volume={66 2},
  pages={91-5}
}
An enzyme-linked immunosorbent assay (ELISA) for hippuric acid (HA) was developed using polyclonal anti-HA antibodies. Anti-HA antibodies were obtained by immunizing rabbits with N-benzoyl-cysteine (B-Cys) or N-alpha-benzoyl-lysine (B-Lys). An antibody with highest reactivity to HA was obtained from anti-B-Lys antiserum by affinity chromatography with B-Cys-Sepharose. The ELISA system was composed of solid-phase B-Cys, anti-HA antibody, and horseradish peroxidase-conjugated anti-rabbit… CONTINUE READING

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