An enzyme immunoassay for salmon gonadotropin-releasing hormone and its application to the study of the effects of diet on brain and pituitary GnRH in the sea bass, Dicentrarchus labrax.

@article{Kah1994AnEI,
  title={An enzyme immunoassay for salmon gonadotropin-releasing hormone and its application to the study of the effects of diet on brain and pituitary GnRH in the sea bass, Dicentrarchus labrax.},
  author={Olivier Kah and Silvia Zanuy and Philippe Pradelles and J De La Cerda and Manuel Carrillo},
  journal={General and comparative endocrinology},
  year={1994},
  volume={95 3},
  pages={464-74}
}
The effects of two different diets [diet 1 (D1), high protein-low carbohydrate; diet 2 (D2), low protein-high carbohydrate] on brain and pituitary gonadotropin-releasing hormone (GnRH) contents, as well as circulating steroids and vitellogenin, were studied over the reproductive period of the sea bass. Salmon GnRH was measured using a newly developed competitive enzyme immunoassay with an enzymatic tracer made of sGnRH covalently coupled to acetylcholinesterase from the electric organ of the… CONTINUE READING

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