An enzyme immunoassay for rat growth hormone: validation and application to the determination of plasma levels and in vitro release.

Abstract

A competitive enzyme immunoassay for rat growth hormone (rGH) has been developed using polyclonal anti-rGH antibodies and an acetylcholinesterase (EC 3.1.1.7.) enzymatic tracer coupled covalently with rGH. The assay was performed in 96-well microtiter plates coated with rabbit polyclonal anti-goat immunoglobulin antibodies. Molecular sieve filtration and… (More)

Topics

Cite this paper

@article{Ezan1997AnEI, title={An enzyme immunoassay for rat growth hormone: validation and application to the determination of plasma levels and in vitro release.}, author={Eric Ezan and E. Laplante and Marie-Th{\'e}r{\`e}se Bluet-Pajot and Françoise Mounier and Serhat Mamaş and Dominique Grouselle and J. M. Grognet and Claude Kordon}, journal={Journal of immunoassay}, year={1997}, volume={18 4}, pages={335-56} }