An enzymatic cycling method for the measurement of myo-inositol in biological samples.

@article{Kouzuma2001AnEC,
  title={An enzymatic cycling method for the measurement of myo-inositol in biological samples.},
  author={Takuji Kouzuma and Mamoru Takahashi and Teruo Endoh and Rina Kaneko and Nobuo Ura and Kazuhiro Shimamoto and Naoki Watanabe},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={2001},
  volume={312 1-2},
  pages={143-51}
}
INTRODUCTION A sensitive and simple enzymatic cycling method is described for the quantitation of myo-inositol in biological samples. METHODS The method involves the use of a sensitive and simple enzymatic cycling method is described for the quantitation of myo-inositol in biological samples. The method involves use of thio-NAD(+), NADH and thermostable myo-inositol dehydrogenase (IDH; EC. 1.1.1.18) and measurement of the increase in absorbance at 405 nm of thio-NADH at 37 degrees C… CONTINUE READING

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