An enzymatic-HPLC assay to monitor endogenous D-serine release from neuronal cultures.

@article{Radzishevsky2012AnEA,
  title={An enzymatic-HPLC assay to monitor endogenous D-serine release from neuronal cultures.},
  author={Inna S Radzishevsky and Herman Wolosker},
  journal={Methods in molecular biology},
  year={2012},
  volume={794},
  pages={
          291-7
        }
}
D-Serine is a transmitter-like molecule that physiologically activates NMDA receptors in the brain. Although D-serine was thought to be exclusively released by astrocytes, we recently demonstrated endogenous D-serine release from neurons in cultures and slices. So far high-performance liquid chromatography (HPLC) has been the standard technique to monitor D-serine and other amino acids. This method employs pre-column derivatization with a chiral reagent to produce fluorescence derivatives that… CONTINUE READING