An enrichment broth culture-duplex PCR combination assay for the rapid detection of enterotoxigenic Clostridium perfringens in fecal specimens.

@article{Tansuphasiri2005AnEB,
  title={An enrichment broth culture-duplex PCR combination assay for the rapid detection of enterotoxigenic Clostridium perfringens in fecal specimens.},
  author={Unchalee Tansuphasiri and Charnchudhi Chanyasanha and Nattasai Cheaochantanakij},
  journal={The Southeast Asian journal of tropical medicine and public health},
  year={2005},
  volume={36 5},
  pages={
          1229-38
        }
}
An enrichment broth culture-duplex PCR combination assay was devised to identify Clostridium perfringens directly from fecal samples. The method consists of a combination of short enrichment of samples in selective media, DNA isolation, and performing duplex PCR using two pairs of primers which identify C. perfringens strains that harbor the virulence enterotoxin gene. Comparison of two selective enrichment media and two incubation temperatures showed that the reinforced clostridial medium with… CONTINUE READING

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