An enhancement method for immunohistochemical staining of proliferating cell nuclear antigen in archival rodent tissues.

@article{Greenwell1991AnEM,
  title={An enhancement method for immunohistochemical staining of proliferating cell nuclear antigen in archival rodent tissues.},
  author={Allison C Greenwell and Julie F Foley and Robert R. Maronpot},
  journal={Cancer letters},
  year={1991},
  volume={59 3},
  pages={251-6}
}
An enhanced immunohistochemical procedure to detect proliferating cell nuclear antigen (PCNA), an endogenous cell replication marker, has been successfully applied to formalin-fixed, paraffin-embedded archival rat and mouse tissues. The procedure involves microwave oven heating of tissue sections in a commercially available antigen retrieval solution of heavy metal salts. Successful immunohistochemical staining of PCNA can be consistently obtained in tissues fixed for over 24 months in formalin… CONTINUE READING