An enhanced system for unnatural amino acid mutagenesis in E. coli.

@article{Young2010AnES,
  title={An enhanced system for unnatural amino acid mutagenesis in E. coli.},
  author={T. Young and Insha Ahmad and Jun Yin and P. Schultz},
  journal={Journal of molecular biology},
  year={2010},
  volume={395 2},
  pages={
          361-74
        }
}
We report a new vector, pEVOL, for the incorporation of unnatural amino acids into proteins in Escherichia coli using evolved Methanocaldococcus jannaschii aminoacyl-tRNA synthetase(s) (aaRS)/suppressor tRNA pairs. [...] Key Result This new system affords higher yields of mutant proteins through the use of both constitutive and inducible promoters to drive the transcription of two copies of the M. jannaschii aaRS gene.Expand
An efficient system for incorporation of unnatural amino acids in response to the four-base codon AGGA in Escherichia coli.
Reassigning Sense Codon AGA to Encode Noncanonical Amino Acids in Escherichia coli
Introducing novel protein functionality using unnatural amino acids
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