An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes.

@article{Berryman1990AnEM,
  title={An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes.},
  author={Mark A. Berryman and R D Rodewald},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1990},
  volume={38 2},
  pages={159-70}
}
We have devised a method for immunogold staining of unosmicated, plastic-embedded tissue which gives high levels of specific staining without scrificing cell ultrastructure. The key to this method is a combination of several standard techniques optimized to preserve cell membranes as well as antigen. Important conditions include (a) a combination primary fixative, (b) post-fixation with uranyl acetate to preserve membrane phospholipids, (c) dehydration with acetone to minimize extraction of… CONTINUE READING
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