An enhanced mass spectrometry approach reveals human embryonic stem cell growth factors in culture.

@article{Bendall2009AnEM,
  title={An enhanced mass spectrometry approach reveals human embryonic stem cell growth factors in culture.},
  author={Sean C Bendall and Chris G Hughes and J Larry Campbell and Morag H. Stewart and Paula Pittock and Suya Liu and {\'E}ric Bonneil and Pierre Thibault and Mickie Bhatia and Gilles A. Lajoie},
  journal={Molecular & cellular proteomics : MCP},
  year={2009},
  volume={8 3},
  pages={421-32}
}
The derivation and long-term maintenance of human embryonic stem cells (hESCs) has been established in culture formats that are both dependent and independent of support (feeder) cells. However, the factors responsible for preserving the viability of hESCs in a nascent state remain unknown. We describe a mass spectrometry-based method for probing the secretome of the hESC culture microenvironment to identify potential regulating protein factors that are in low abundance. Individual samples were… CONTINUE READING