An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells.

@article{Zhang1996AnEG,
  title={An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells.},
  author={Guisheng Zhang and V. Gurtu and Steven R. Kain},
  journal={Biochemical and biophysical research communications},
  year={1996},
  volume={227 3},
  pages={707-11}
}
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has become an important marker of gene expression. However, the sensitivity of wild-type GFP has been below that of standard reporter proteins, such as beta-galactosidase, which utilize enzymatic amplification. To improve the detection of GFP in transfected mammalian cells, we have constructed a unique GFP variant which contains chromophore mutations that make the protein 35 times brighter than wild-type GFP, and is codon… CONTINUE READING
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