An enhanced gene targeting toolkit for Drosophila: Golic+.

@article{Chen2015AnEG,
  title={An enhanced gene targeting toolkit for Drosophila: Golic+.},
  author={H M Chen and Yaling Huang and Barret D. Pfeiffer and Xiaohao Yao and Tzumin Lee},
  journal={Genetics},
  year={2015},
  volume={199 3},
  pages={683-94}
}
Ends-out gene targeting allows seamless replacement of endogenous genes with engineered DNA fragments by homologous recombination, thus creating designer "genes" in the endogenous locus. Conventional gene targeting in Drosophila involves targeting with the preintegrated donor DNA in the larval primordial germ cells. Here we report G: ene targeting during O: ogenesis with L: ethality I: nhibitor and C: RISPR/Cas (Golic+), which improves on all major steps in such transgene-based gene targeting… CONTINUE READING
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