An engineered extraplastidial pathway for carotenoid biofortification of leaves

  title={An engineered extraplastidial pathway for carotenoid biofortification of leaves},
  author={Trine Bundgaard Andersen and Briardo Llorente and Luca Morelli and Salvador Torres-Montilla and Guillermo Bordanaba-Florit and Fausto A Espinosa and Mar{\'i}a Rosa Rodr{\'i}guez-Goberna and Bego{\~n}a Olmedilla-Alonso and Manuel J. Llansola-Portoles and Andrew A Pascal and Manuel Rodr{\'i}guez-Concepc{\'i}on},
  journal={Plant Biotechnology Journal},
  pages={1008 - 1021}
Summary Carotenoids are lipophilic plastidial isoprenoids highly valued as nutrients and natural pigments. A correct balance of chlorophylls and carotenoids is required for photosynthesis and therefore highly regulated, making carotenoid enrichment of green tissues challenging. Here we show that leaf carotenoid levels can be boosted through engineering their biosynthesis outside the chloroplast. Transient expression experiments in Nicotiana benthamiana leaves indicated that high extraplastidial… 

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