An engineered Escherichia coli having a high intracellular level of ATP and enhanced recombinant protein production

@article{Kim2011AnEE,
  title={An engineered Escherichia coli having a high intracellular level of ATP and enhanced recombinant protein production},
  author={Hye-Jung Kim and Yeong Deok Kwon and Sang Yup Lee and Pil Kim},
  journal={Applied Microbiology and Biotechnology},
  year={2011},
  volume={94},
  pages={1079-1086}
}
Artificial amplification of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) under glycolytic conditions enables Escherichia coli to maintain a greater intracellular ATP concentration during its growth phase. To demonstrate the biotechnological benefit of E. coli harboring a high intracellular ATP concentration, we compared the recombinant protein synthesis of a soluble protein (enhanced green fluorescence protein, GFP) with that of a secretory protein (alkaline protease, AP), under… CONTINUE READING

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